The amino acid sequence of the tryptic peptides isolated from dogfish M4 lactate dehydrogenase.
نویسندگان
چکیده
The peptides which result from treatment of the S-[14C]carboxymethyl derivative of dogfish M4 lactate dehydrogenase (EC 1.1.1.27) with trypsin have been isolated and their sequences have been elucidated. Each identical subunit has a molecular weight of 36,000 and on the basis of the amino acid composition 40 unique tryptic peptides are anticipated. Thirty-seven of these peptides have been isolated and have been completely characterized. The amino acid sequences of these peptides are presented.
منابع مشابه
The subunits of dogfish M4 lactic dehydrogenase.
Treatment of dogfish l& lactic dehydrogenase with carboxypeptidase A releases 1 mole of phenylalanine per 36,000 g of the enzyme and no other amino acids. A mixture of carboxypeptidases A and B releases 1 mole of phenylalanine and 1 mole of lysine per 36,000 g of the lactic dehydrogenase. The amino terminus of dogfish M4 lactic dehydrogenase does not react with either dansyl chloride or the Edm...
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Amino acid sequence analysis was carried out on the so-called 'insoluble tryptic • @ peptide(ƒ¿T12,13)'which • @ was • @precipitated • @ when • @the • @tryptic • @ hydro-lysate • @ of • @ the • @ƒ¿-polypeptide • @ chain • @in • @macaca • @ mulatta • @ monkey • @ hemoglobin • @ was adjusted to pH 6.4. This peptide was oxidized with performic acid and then was hydrolyzed with chymotrypsin. The ch...
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The effect of reversible inactivation on the physical and chemical properties of dogfish M4 and chicken heart H* lactate dehydrogenases has been studied. Reduced diphosphopyridine nucleotide was found to have a significant effect on the rate of recovery of the enzymatic activity, but not on the over-all recovery. Optical rotatory dispersion, circular dichroism, fluorescence, and immunological s...
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 250 22 شماره
صفحات -
تاریخ انتشار 1975